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Copyright © 2006 by Eric Martz
(University of Massachusetts, Amherst MA USA) and
Fadel Samatey, Nanobiology Group, Osaka University, Japan.
Permission is hereby granted to use this document, or portions thereof, for non-profit educational purposes, provided this copyright statement remains intact within this document, or any portions or derivatives. Other uses require explicit permission from the author(s).

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Contacts Between Chains in the Bacterial Flagellar Hook
by Eric Martz, Fadel Samatey, Katsumi Imada, and Keiichi Namba

A chapter in The Bacterial Flagellar Hook: A Molecular Universal Joint, based on Samatey et al., Nature 431:1062-1068, 2004.
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Straight Model of the Flagellar Hook

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Seven monomers extracted from the straight model of the flagellar hook.

The central (white) monomer experiences all the types of contacts that occur in the straight model.
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Contacts between monomers in the straight model of the flagellar hook.

The white monomer chain is decorated with all atoms on neighboring chains that are within 4 Å. Contacting atoms are colored by chain.
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Contacts between monomers in the straight model of the flagellar hook.

Here the central chain is shown as a backbone trace. Balls represent all atoms on neighboring chains that are likely to be non-covalently bonded (putative hydrogen bonds to 3.5 Å and van der Waals interactions to 4.5 Å). Sticks represent bonds within 7 Å of contacting atoms. Contacting atoms are colored by chain.
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Contacts between monomers in the straight model of the flagellar hook: Contacting elements.

Same as the previous slide except that balls and sticks are colored by element. C O N S
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Contacts between monomers in the straight model of the flagellar hook: Contacting and contacted elements.

Same as the previous slide except that atoms in the central chain that are contacted by neighboring chains are shown as balls with dot surfaces. C O N S
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Contacts between monomers in the straight model of the flagellar hook: Conservation I.

Same as the previous slides except that contacting atoms between chains are colored by evolutionary conservation. Surprisingly, and unlike the contacts in the flagellar filament, the hook contacts are poorly conserved. There may be higher conservation in the 26% of FlgE that is missing in this crystal structure, and in the straight filament model.
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Contacts between monomers in the straight model of the flagellar hook: Conservation II.

Same as the previous slide, except that the central monomer chain is spacefilled, and all atoms are colored by evolutionary conservation.
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Technical notes:

Monomer is Protein Data Bank accession 1WLG.
ConSurf colors from 158 Uniprot sequences (E < 10^-13), run 1151925203.

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